In vitro Antimicrobial Activity Screening of Punica Granatum Extracts Against Human Pathogens
Fisseha Alemu, Addisu Tilahun and
Correspondence Address :
College of Natural Science
Institute of Biotechnology
Received on: June 01, 2017, Accepted on: July 21, 2017, Published on: July 31, 2017
Citation: Fisseha Alemu, Addisu Tilahun, Esayas Elias (2017). In Vitro Antimicrobial Activity Screening of Punica Granatum Extracts Against Human Pathogens
Copyright: 2017 Fisseha Alemu, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The demand for new antibiotics continues to grow due to the rapid spread of antibioticresistant pathogens causing life-threatening infections. Currently, many scientists devoted in searching new biologically active antimicrobials which have possibly novel mechanisms of action, with no many side effects and wide therapeutic potentials. The study was done to evaluate the antimicrobial potentials of Punica granatum leaf extract against standard and drug resistant bacteria and Cadidia albicans using agar well diffusion assay and broth dilution methods. Active compounds of these plants which are responsible for the antimicrobial activities were extracted using four different solvents (acetone, methanol, chloroform and water). Vancomycin, methicillin, and amoxicillin were used as positive controls and pure water was as negative control in the well diffusion assay. Leaf crude extracts of the plant were showed various degrees of antimicrobial activities with the maximum mean inhibition zones reaches to 22.6 +/- 5.50 mm in diameter with methanol leaf extracts against methicillin resistant Staphylococcus aureus. All the test organisms which were inhibited by all the extracts in agar well diffusion method also showed minimum inhibitory concentration values at concentration ranging from 6.25% to 50%. From all the test organisms used, Punica granatum relatively demonstrated better potential with minimum inhibitory concentration between 6.25% and 12% for all its extracts against on both standard and drug resistant Streptococcus pneumonia. Moreover, the most frequent minimum inhibitory concentration value among all extracts was 6.25%, followed 12.5%, 25% and 50%. Synergetic effects were obtained against methicillin resistant Staphylococcus aureus with the combined effects between chloroform extracts of Punica granatum with that of amoxicillin and vancomycin standard antibiotics. Results of the present investigation verified that the therapeutic potentials of Punica granatum leaf extracts to combat the raise of drug resistant bacteria and fungus hence could be proposed as an alternative approaches for the discovery of novel antimicrobial drugs.
Keywords: Crude extract; Synergetic effect; Drug resistant; Zone of inhibition