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Khalid Aftab

EDITORIAL BOARD MEMBER - Khalid Aftab

Khalid Aftab

University of Karachi
Pakistan

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Holarrhena pubescens belongs to the family Apocynacea, commonly known as “Kurchi” is highly reputed in traditional medicine as a remedy for amoebic dysentery and other intestinal ailment. Bioassay-directed fractionation of the ethanolic extract of Holarrhena pubescens resulted in the isolation of steroidal alkaloids (Holamide and Pubscinine). In anaesthetized rats, the Holamide and Pubscinine caused a fall in blood pressure in a dose-dependent manner. Pretreatment of animals Atropine completely abolished the hypotensive response of Acetycholine; whereas hypotensive effect of Holamide and Pubscinine were not modified by Atropine. Similarly Acetylcholine produced contractile effect in guinea-pig ileum, which was antagonized by atropine; however both (Holamide and Pubscinine) failed to produce any stimulant response on guinea-pig ileum. These data indicate that the steroidal alkaloids i.e. Holamide and Pubscinine from Holarrhena pubescens mediated hypotensive response through a mechanism different to that of Acetycholine.

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